Platelet Glycoproteins LIb and Lila Associated With Blood Monocytes Are Derived From Platelets
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چکیده
Platelet glycoprotein lIb/Illa (GP lIb/Illa). the receptor complex for fibrinogen, has been regarded as a megakaryocyte/platelet lineage-restricted antigen. Recently. however. it has been reported that GP lIb/Illa is expressed in blood monocytes. Studies were performed to establish the origin and immunological characteristics of monocyteassociated glycoproteins lIb and lIla (GPs lIb and lIla). Preparations of blood monocytes containing varying platelet-monocyte ratios were metabolically labeled with r5Sjmethionine with the expectation that any newly synthesized GPs lIb and lIla would be monocyte-derived. since platelets have only rudimentary protein synthetic apparatuses. Analyses of sodium dodecyl sulfate (SDS) gels of homogenates of cell preparations containing from 200 to 5:1 platelet-monocyte ratios revealed that unlabeled GPs llb and lIla were readily immunoisolated using protein
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Isolation and Structural Characterization of the Polypeptide Subunits of Membrane Glycoprotein lib-Illa From Human Platelets
We have previously demonstrated the isolation of platelet membrane glycoprotein lIb-Illa by affinity chromatography with a specific monoclonal antibody.1 We have now separated the polypeptide subunits lIb and lIla of the isolated glycoprotein by preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis and have compared their structural features. Both llb and lIla contain -.1 5% car...
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